Histopathological study reveals new insights into responses of chestnut (Castanea spp.) to root infection by Phytophthora cinnamomi.
Identifieur interne : 000170 ( Main/Exploration ); précédent : 000169; suivant : 000171Histopathological study reveals new insights into responses of chestnut (Castanea spp.) to root infection by Phytophthora cinnamomi.
Auteurs : Patrícia Fernandes [Portugal] ; Maria Helena Machado [Portugal] ; Maria Do Ceu Silva [Portugal, Arabie saoudite] ; Rita Lourenco Costa [Portugal]Source :
- Phytopathology [ 0031-949X ] ; 2020.
Abstract
The European chestnut (Castanea sativa) is threatened by the hemibiotrophic oomycete Phytophthora cinnamomi, the causal agent of ink disease. Chestnut species have different susceptibility levels to P. cinnamomi, with the Asian species (C. crenata; C. mollissima) exhibiting the highest level of resistance. A histological approach was used to study the responses exhibited by susceptible and resistant chestnut genotypes by characterizing the early stages of P. cinnamomi infection and the cellular responses it induces in roots. C. sativa (susceptible) and C. crenata (resistant) plantlets, were inoculated with a P. cinnamomi virulent isolate with a zoospore suspension or by direct contact with mycelia agar plugs. Root samples were collected at 0.5, 3.5, 24, 48, and 72 hours after inoculation (hai) for microscopic observations. Penetration was observed, in both species, at 0.5hai and 3.5hai with mycelium and zoospore inoculations, respectively. In both inoculation methods, following the penetration into the rhizodermis, P. cinnamomi hyphae grew inter- and intracellularly through the cortex and into the vascular cylinder. C. crenata cells displayed a delay in the pattern of infection, by having fewer cell layers colonized when compared with C. sativa. At 72hai, the collapse of the first layers of C. sativa cortical cells was observed, indicating the beginning of necrotrophy. C. crenata was able to respond more efficiently to P. cinnamomi than C. sativa, by restricting the pathogen's growth area through the early activation of resistance responses, such as callose deposition around some intracellular hyphae, HR-like cell death, cell wall thickening and accumulation of phenolic-like compounds.
DOI: 10.1094/PHYTO-04-20-0115-R
PubMed: 32755337
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Chestnut species have different susceptibility levels to P. cinnamomi, with the Asian species (C. crenata; C. mollissima) exhibiting the highest level of resistance. A histological approach was used to study the responses exhibited by susceptible and resistant chestnut genotypes by characterizing the early stages of P. cinnamomi infection and the cellular responses it induces in roots. C. sativa (susceptible) and C. crenata (resistant) plantlets, were inoculated with a P. cinnamomi virulent isolate with a zoospore suspension or by direct contact with mycelia agar plugs. Root samples were collected at 0.5, 3.5, 24, 48, and 72 hours after inoculation (hai) for microscopic observations. Penetration was observed, in both species, at 0.5hai and 3.5hai with mycelium and zoospore inoculations, respectively. In both inoculation methods, following the penetration into the rhizodermis, P. cinnamomi hyphae grew inter- and intracellularly through the cortex and into the vascular cylinder. C. crenata cells displayed a delay in the pattern of infection, by having fewer cell layers colonized when compared with C. sativa. At 72hai, the collapse of the first layers of C. sativa cortical cells was observed, indicating the beginning of necrotrophy. C. crenata was able to respond more efficiently to P. cinnamomi than C. sativa, by restricting the pathogen's growth area through the early activation of resistance responses, such as callose deposition around some intracellular hyphae, HR-like cell death, cell wall thickening and accumulation of phenolic-like compounds.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32755337</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2020</Year><Month>Aug</Month><Day>05</Day></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Histopathological study reveals new insights into responses of chestnut (Castanea spp.) to root infection by Phytophthora cinnamomi.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-04-20-0115-R</ELocationID><Abstract><AbstractText>The European chestnut (Castanea sativa) is threatened by the hemibiotrophic oomycete Phytophthora cinnamomi, the causal agent of ink disease. Chestnut species have different susceptibility levels to P. cinnamomi, with the Asian species (C. crenata; C. mollissima) exhibiting the highest level of resistance. A histological approach was used to study the responses exhibited by susceptible and resistant chestnut genotypes by characterizing the early stages of P. cinnamomi infection and the cellular responses it induces in roots. C. sativa (susceptible) and C. crenata (resistant) plantlets, were inoculated with a P. cinnamomi virulent isolate with a zoospore suspension or by direct contact with mycelia agar plugs. Root samples were collected at 0.5, 3.5, 24, 48, and 72 hours after inoculation (hai) for microscopic observations. Penetration was observed, in both species, at 0.5hai and 3.5hai with mycelium and zoospore inoculations, respectively. In both inoculation methods, following the penetration into the rhizodermis, P. cinnamomi hyphae grew inter- and intracellularly through the cortex and into the vascular cylinder. C. crenata cells displayed a delay in the pattern of infection, by having fewer cell layers colonized when compared with C. sativa. At 72hai, the collapse of the first layers of C. sativa cortical cells was observed, indicating the beginning of necrotrophy. C. crenata was able to respond more efficiently to P. cinnamomi than C. sativa, by restricting the pathogen's growth area through the early activation of resistance responses, such as callose deposition around some intracellular hyphae, HR-like cell death, cell wall thickening and accumulation of phenolic-like compounds.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fernandes</LastName><ForeName>Patrícia</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigação Agrária e Veterinária, 112115, Oeiras, Oeiras, Portugal.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Universidade Nova de Lisboa, 50106, Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit),, Oeiras, Lisboa, Portugal; patricia.fernandes@iniav.pt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Machado</LastName><ForeName>Maria Helena</ForeName><Initials>MH</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigacao Agraria e Veterinaria, 112115, Oeiras, Lisboa, Portugal; helena.machado@iniav.pt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva</LastName><ForeName>Maria do Ceu</ForeName><Initials>MDC</Initials><AffiliationInfo><Affiliation>Universidade de Lisboa Instituto Superior de Agronomia, 56054, Centro de Investigação das Ferrugens do Cafeeiro, Lisboa, Lisboa, Portugal.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Universidade de Lisboa Instituto Superior de Agronomia, 56054, Linking Landscape, Environment, Agriculture and Food, Lisboa, Lisboa, Portugal; mariaceusilva@isa.ulisboa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Costa</LastName><ForeName>Rita Lourenco</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Instituto Nacional de Investigacao Agraria e Veterinaria, 112115, Oeiras, Lisboa, Portugal.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Universidade de Lisboa Centro de Estudos Florestais, 451038, Lisboa, Lisboa, Portugal; rita.lcosta@iniav.pt.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Disease Control and Pest Management</Keyword><Keyword MajorTopicYN="N">Genetics and resistance</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32755337</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-04-20-0115-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Arabie saoudite</li><li>Portugal</li></country></list><tree><country name="Portugal"><noRegion><name sortKey="Fernandes, Patricia" sort="Fernandes, Patricia" uniqKey="Fernandes P" first="Patrícia" last="Fernandes">Patrícia Fernandes</name></noRegion><name sortKey="Costa, Rita Lourenco" sort="Costa, Rita Lourenco" uniqKey="Costa R" first="Rita Lourenco" last="Costa">Rita Lourenco Costa</name><name sortKey="Costa, Rita Lourenco" sort="Costa, Rita Lourenco" uniqKey="Costa R" first="Rita Lourenco" last="Costa">Rita Lourenco Costa</name><name sortKey="Fernandes, Patricia" sort="Fernandes, Patricia" uniqKey="Fernandes P" first="Patrícia" last="Fernandes">Patrícia Fernandes</name><name sortKey="Machado, Maria Helena" sort="Machado, Maria Helena" uniqKey="Machado M" first="Maria Helena" last="Machado">Maria Helena Machado</name><name sortKey="Silva, Maria Do Ceu" sort="Silva, Maria Do Ceu" uniqKey="Silva M" first="Maria Do Ceu" last="Silva">Maria Do Ceu Silva</name></country><country name="Arabie saoudite"><noRegion><name sortKey="Silva, Maria Do Ceu" sort="Silva, Maria Do Ceu" uniqKey="Silva M" first="Maria Do Ceu" last="Silva">Maria Do Ceu Silva</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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